Fig. 8.

LTP is associated with a unique peak of autonomous PKC activity that elutes from DEAE columns. Shown are elution profiles of autonomous PKC activity from either control slices (open circles in A; open squares inB) or slices taken 2 min (filled circles in A) and 45 min (filled squares in B) after the final train of LTP-inducing HFS. Either 2 min (n = 5) or 45 min (n = 5) after the final train of HFS the slices were frozen, and the area between the stimulating and recording electrodes was dissected and homogenized. The soluble fraction of the homogenates was applied to mini-DEAE columns, and PKC was eluted with either 0.1 or 0.25 m NaCl (arrows). Autonomous PKC activity was measured as described in Materials and Methods.