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. 2002 Feb 1;22(3):757–765. doi: 10.1523/JNEUROSCI.22-03-00757.2002

Fig. 9.

Fig. 9.

β-Catenin facilitates the accumulation of the C-terminal region of S-SCAM at synapses. A, Myc–S-SCAM-10 containing PDZ4 and PDZ5 in a primary cultured hippocampal neuron. Neurons expressing Myc–S-SCAM-10 were stained with the polyclonal sheep anti-Myc and the anti-synaptophysin antibodies. The distribution of Myc–S-SCAM-10 was similar to that of GFP–S-SCAM-10. The number of clusters formed by Myc–S-SCAM-10 was small. a, Myc–S-SCAM-10 (arrows);b, synaptophysin (arrowheads);c, a superimposed image of Myc–S-SCAM-10 and synaptophysin. Scale bar, 10 μm. B, Myc–S-SCAM-10 coexpressed with GFP–β-catenin-1. Primary cultured hippocampal neurons expressing Myc–S-SCAM-10 and GFP–β-catenin-1 were immunostained with the sheep anti-Myc and the mouse anti-synaptophysin antibodies. a, Merged image of GFP–β-catenin-1 (triangles) and synaptophysin (arrowheads); b, merged image of Myc–S-SCAM-10 (arrows) and synaptophysin (arrowheads). Scale bar, 1 μm.