Fig. 3.
Effect of the addition of microglia to neuron-enriched cultures on rotenone-induced degeneration of dopaminergic neurons. Neuron-enriched mesencephalic cultures were supplemented with 2.5–7.5 × 104microglia/well. At 24 hr later the cultures were treated with the vehicle or 10 nm rotenone, and DA uptake was determined 8 d after the treatment. The results are expressed as a percentage of the control cultures and are the mean ± SEM of three experiments performed in triplicate. *p < 0.01, compared with the control.