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. 2019 Sep 13;8(1):1347–1360. doi: 10.1080/22221751.2019.1662736

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© 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group, on behalf of Shanghai Shangyixun Cultural Communication Co., Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — SMYD3 specifically interacted with EBOV NP. (A) Endogenous SMYD3 was co-purified with NP. Plasmids encoding GFP-HA, VP35-HA, VP30-HA and NP-HA were separately transfected into HEK293T cells. Cells were collected 48 h p.t., followed by anti-HA co-IP assay and western blotting. Representative results of 3 independent experiments are shown. (B) SMYD3 was recruited to EBOV inclusion bodies by NP. HEK293T cells were transfected with empty vector (panel i) or plasmids encoding NP-HA, VP35, VP30 and L (panel ii), NP-HA (panel iii), VP35-HA (panel iv) or VP30-HA (panel v). At 48 h p.t., cells were fixed with 4% poly-formaldehyde, followed by Immunofluorescence assay (IFA). Scale bar, 10 μm.