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. 2002 Dec 1;22(23):10201–10208. doi: 10.1523/JNEUROSCI.22-23-10201.2002

Fig. 2.

Fig. 2.

Effect of trophic factor-neutralizing antisera on KCa channel expression in developing LMNs.A, Coculture of E8 LMNs with hindlimb myotubes for 24 hr allowed for a significant developmental increase in the functional expression of KCa channels. The stimulatory effect of hindlimb myotubes on KCa channel expression was reduced by incubation with neutralizing antisera specific for GDNF but not NT-4.B, GDNF-neutralizing antiserum did not affect the density of Ca2+ currents in LMNs cocultured for 24 hr with hindlimb myotubes. C, The stimulatory effect of 24 hr treatment with MEX on KCa channel expression in E8 LMNs was also reduced by overnight incubation of MEX with neutralizing antisera specific for GDNF but not NT-4. Neutralizing antisera were used at 10 μg/ml. D, Reduction in the functional expression of KCa channels in LMNs developing in ovo after hindlimb injections of the microtubule inhibitor colchicine or GDNF-neutralizing antiserum. Both of these treatments evoked a comparable reduction in KCa expression.