Fig. 1.
Short-term patterned electrical stimulation, but not short-term continuous KCl depolarization, significantly increases release of native BDNF from hippocampal neurons. Mean levels of BDNF measured in sister cultures of newborn hippocampal neurons after 3 d in vitro plus 30 min of the following: (1) control conditions (no stimulation; n = 48), (2) TBS (25 bursts of 4 pulses at 100 Hz, delivered at 5 Hz, every 20 sec;n = 30), or (3) continuous depolarization with 50 mm KCl (n = 39). ***p < 0.001; n.s., not significant.