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Activity-dependent release of native BDNF depends on action potentials. Mean levels of BDNF released in sister cultures of newborn hippocampal neurons during 30 min of electrical field stimulation [100 biphasic rectangular pulses of 4 msec, delivered at 25 Hz (n = 12), 100 Hz (n = 10), and TBS (n = 4), once every 20 sec] in the absence (black bars) or presence (gray bars) of 1.5 μm TTX, an inhibitor of voltage-dependent sodium channels. **p < 0.01; ***p < 0.001.
