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. 2002 Dec 1;22(23):10399–10407. doi: 10.1523/JNEUROSCI.22-23-10399.2002

Fig. 5.

Fig. 5.

Release of native BDNF evoked by patterned electrical stimulation requires calcium influx through N-type voltage-activated calcium channels. Mean levels of BDNF released in sister cultures of newborn hippocampal neurons during 30 min of electrical field stimulation (100 biphasic rectangular pulses of 4 msec, delivered at 25 Hz, 100 Hz, and TBS, once every 20 sec):A, in the presence (Control; black bars) or absence (Ca-free; gray bars) of extracellular calcium (25 Hz, n = 6; 100 Hz, n = 4; TBS, n = 4);B, in the absence (Control; black bars) or presence of 1 μm ω-Conotoxin GVIA, an N-type channel antagonist (ω-Conotoxin; gray bars) (25 Hz, n = 4; 100 Hz,n = 10; TBS, n = 4);C, in the absence (Control; black bars) or presence of 2 μm nimodipine, an L-type channel antagonist (Nimodipine; gray bars) (25 Hz, n = 4; 100 Hz,n = 4; TBS, n = 6). **p < 0.01; ***p < 0.001;n.s., not significant.