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Competition EMSA of WT49 probe with the chick pineal nuclear proteins. Before mixing with end-labeled WT49 probe, pineal nuclear proteins prepared from light-adapted chicks were preincubated with the competitor shown on the top of each lane. The sequences of M1–M8 are shown in Figure2A; WT49 represents DNA fragment −1122 to −1074 of the pinopsin promoter region. An arrowheadindicates the position of a band representing an LRE-specific interaction.
