Fig. 4.

Transcriptional repression through thepinopsin LRE (−1122 to −1074). A, Schematic of the promoter/reporter constructs used in this experiment.luc, Luciferase. B–D, The reporter activities measured in the chick pineal cells (B), LMH cells (C), and NIH 3T3 cells (D). The cells transfected with each construct were harvested in the light (open bars inB) or in the dark (filled bars inB–D) to measure the luciferase activities. A typical set of data from three independent experiments is shown inB–D. Error bars indicate means ± SD of three replicated cell cultures.