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. 2002 Jun 1;22(11):4357–4363. doi: 10.1523/JNEUROSCI.22-11-04357.2002

Fig. 5.

Fig. 5.

Characterization of the pinopsinLRE-binding protein by EMSA. A, EMSA using the WT49 probe in the presence of the pineal nuclear proteins prepared from light- or dark-adapted chicks. B, Competition EMSA with pineal nuclear proteins in the presence of competitors: WT49, M5, DC5, mutDC5, AVP, and mutAVP. C, Competition EMSA with pineal nuclear proteins in the presence of competitors: WT49, cPer2, and mutcPer2. D, Sequence comparison of the vicinity of the E-box region from chickenpinopsin, cPer2, and mouse AVP (mAVP).E, EMSA using the chick pineal and liver nuclear proteins. In each panel, the position of a band representing a specific interaction with the LRE is indicated by anarrowhead.