Table 1.

Percentage aggregation of Cx- and mock-transfected C6 glioma cells

	Percentage of cells in aggregates	Percentage of donor cells transferring dye	Mean number of receiving cells per donor cell	Coupling index
C6-Cx43	72  ± 4	49  ± 4	14  ± 2	7  ± 0.07
C6-Cx40	65  ± 3	55  ± 2	9  ± 1	5  ± 0.2
C6-Cx32	54  ± 3*	89  ± 3	21  ± 3	18  ± 0.1
C6-mock 1	15  ± 4*	13  ± 1.2	1.1  = 0.3	0.14  ± 0.04*
C6-mock 2	13  ± 3*	0.8  ± 0.2	1.1  ± 0.2	0.01  ± 0.005*
C6-mock 3	10  ± 4*	18  ± 1.2	1.2  ± 0.2	0.2  ± 0.1*
C6-WT	18  ± 3*	27  ± 2	3  ± 0.1	0.8  ± 0.2*

Quantitative analysis of short-term aggregation assays. This table lists the percentage of Cx- and mock-transfected C6 cells that form aggregates in suspension assay. Dye transfer assays ofGoldberg et al. (1995) were used as described in Materials and Methods. All assays were performed in a minimum of three independent runs (range, 3–16; mean, 9).

^*, Significantly different from C6-Cx43 cells (p < 0.05). ANOVA for group comparison with post hoc t test is used to establish significant difference between the groups. All numbers indicate ± SEM.