Fig. 6.

Localization of Caskin 1 analyzed by immunocytochemistry. A–C, Adjacent cryostat sections from rat cerebellum stained with antibodies to Rab3A (A) or Caskin 1 (B) or with Caskin 1 preimmune serum (C). The molecular layer (ml), granule cell layer (gl), and deep cerebellar nuclei (dcn) are identified. Scale bar in C, 0.1 mm (applies to A–C). D,E, Cultured hippocampal neurons double-labeled with a monoclonal antibody to synaptophysin (D) and a polyclonal antibody to Caskin 1 (E). Scale bar inE, 40 μm; (applies to D,E).