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Confirmation of the CASK–Caskin 1 interaction by GST pulldowns. Proteins from rat brain homogenates (lane 1) were bound to GST alone (lane 2) or to GST fusion proteins of the N-terminal CaM kinase domain of CASK (lane 3) or of the N-terminal sequences of Mint 1 (lane 4) or of Caskin 1 (lane 5). Bound proteins were analyzed by immunoblotting with antibodies to CASK, Mint 1, and Caskin 1 as indicated. Numbers on theleft indicate positions of molecular weight markers.
