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. 2002 Jun 1;22(11):4274–4285. doi: 10.1523/JNEUROSCI.22-11-04274.2002

Fig. 5.

Fig. 5.

Dystroglycan is not required for the differentiation of neuronal dendritic and axonal compartments and synaptic terminals. Hippocampal neurons were cultured from wild-type (A) or DG lox/lox (B–E) mice, incubated at 7 d in culture with adenovirus-Cre (A, CE) or not (B), and analyzed at 17–19 d. Neurons were immunolabeled for combinations of β−dystroglycan (A1,B1, C1) and synapsin (A2,B2, C2), GAD (D), MAP2 (E1), and NCAM (E2). Synaptic β−dystroglycan clusters (arrowheads) were detected in wild-type neurons treated with adenovirus-Cre (A) and in DG lox/lox neurons not treated with virus (B), but not in most DG lox/lox neurons treated with adenovirus-Cre (C, arrows; representative of 94% of the neurons). Presynaptic terminals (C, arrows) including GABA synapses (D, arrowheads) formed normally in the absence of dystroglycan. Dystroglycan was also not required to elaborate dendrites (arrowheads) and axons (E, arrows). Scale bar, 10 μm.

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