Table 3.
Expression of M146L mutant presenilin-1 transgenes accelerates formation of Aβinsol in (FVB × 129.Tg2576)F1 APP transgene-positive mice
| PS1(M146L) transgene3-a | Aβ40 (pmol/gm) | Aβ42(43) (pmol/gm) | ||
|---|---|---|---|---|
| Aβsol | Aβinsol | Aβsol | Aβinsol | |
| Positive | 59.7 | 15.7 | 9.9 | 47.1 |
| Positive | 57.3 | 15.7 | 9.5 | 61.5 |
| Positive | 68.4 | 171.4 | 9.4 | 200.8 |
| Negative | 41.8 | 1.1 | 3.5 | 0.7 |
| Negative | 41.6 | 1.4 | 3.6 | 0.5 |
| Negative | 50.2 | 1.3 | 4.0 | 0.5 |
Mice were 50–62 d old. APP transgene-negative mice are not comparable because they express only mouse APP, but values for APP transgene-negative mice in the same assay were Aβsol40 = 2.5 ± 0.5, Aβinsol40 = 0.3 ± 0.5, Aβsol42 = 0.37 ± 0.15, and Aβinsol42 = 0.12 ± 0.25. Aβsolis a SDS-soluble Aβ peptide; Aβinsol is a formic acid releasable Aβ peptide, after SDS extraction.
F3-a
All mice were positive for the Tg2576 APP transgene array.