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. 2002 Mar 1;22(5):1752–1762. doi: 10.1523/JNEUROSCI.22-05-01752.2002

Fig. 6.

Fig. 6.

Methyl donor deficiency-induced neuronal death involves misincorporation of uracil into DNA. a, Neurons were exposed for 5 hr to saline (Control), methionine/folic acid-deficient medium (M/F), or 250 μm homocysteine (Hom) and then were subjected to comet analysis of DNA damage via UDG treatment. Next the nuclei were subjected to comet analysis of DNA damage. Values are the mean and SD (n = 6). *p < 0.01, **p < 0.001 compared with control value and with the corresponding buffer value. b, Cultures were exposed for 48 hr to saline (Control), methionine/folic acid-deficient medium (M/F), 15 μm thymidine plus 30 μm hypoxanthine (Th+H), or the combination of methionine/folic acid-deficient medium plus Th+H. Neuron survival was quantified; the values are the mean and SD (n = 6). **p < 0.01 compared with control;#p < 0.05 compared with the methionine/folic acid-deficient value.