Fig. 11.

Suppression of MMT-induced apoptosis in PC12 cells. A, ROS inhibitors, SOD and MnTBAP.B, Caspase-3 inhibitors, Z-VAD-FMK and Z-DEVD-FMK and PKCδ inhibitor, rottlerin. Subconfluent cultures of undifferentiated PC12 cells were treated with MMT (200 μm) with or without the inclusion of the following inhibitors: ROS inhibitors SOD (100 U/ml) or MnTBAP (10 μm); caspase inhibitors Z-VAD-FMK (100 μm) or Z-DEVD-FMK (50 μm); and PKCδ inhibitor rottlerin (10 μm). Inhibitors were added 30 min before addition of MMT. Cells were harvested 1 hr after MMT treatment. Apoptosis was assayed using ELISA assay as described in Materials and Methods. The data are expressed as percentage of apoptosis observed in vehicle-treated cells. The data represent the mean ± SEM of six individual measurements from three separate experiments. Asterisks (*p < 0.01) indicate significant differences when compared with cells exposed to 200 μm MMT.