Fig. 8.

Colchicine-evoked microtubule depolymerization inhibits PLCβ signaling in SK-N-SH neuroblastoma cells.A, Confocal immunofluorescence image of an SK-N-SH cell treated for 15 min with 33 μm colchicine as described in Materials and Methods. Microtubule depolymerization as well as colocalization (yellow) of tubulin (green) with PIP₂(red) is demonstrated. B,Myo-[³H]inositol-prelabeled SK-N-SH cells were treated for 15 min with 33 μm colchicine (col). Carbachol (carb; 10 μm) was added, the samples were incubated for 30 min at 37°C, and the total inositol phosphate production was measured as described. **Significantly different from control cells (p < 0.01); *significantly different from carbachol-treated cells (p < 0.05).