A single pulse of Ine-P1 expression within 6 hr of eclosion is sufficient to rescue the downturned wings phenotype ofSh; ine double mutants. A, Representative flies of Sh; ine+, Sh; ine1, Sh; ine1; hs-ine-RA (without heat shock), and Sh; ine1;hs-ine-RA (with heat shock), showing the downturned wings phenotype of Sh; ine double mutant and rescue of this phenotype by expression of Ine-P1 from thehs-ine-RA construct. B, Quantification of the heat shock rescue experiments. n = 200 forSh; ine+,n = 80 for Sh; ine1 without heat shock;n = 132 for Sh; ine1; hs-ine-RA without heat shock; n = 85 for Sh; ine1 with heat shock; n = 178 for Sh; ine1;hs-ine-RA with heat shock. Error bars represent SEMs. *p < 0.001 versus Sh; ine1.