Figure 2.
Intrinsic physiological properties of L3PNs assessed in current-clamp recordings in DLPFC or PPC brain slices. The recorded neurons were classified as RS-L3PNs (“RS”) or B-L3PNs (“B”), using iF1–2 versus current plots as illustrated in Figure 1C. The membrane properties reported in the figure were measured as described in Materials and Methods. Shown are the results of one-way ANOVA, or nonparametric Friedman's ANOVA followed by post hoc contrasts. For two parameters (membrane time constant and rheobase), normality tests showed significant deviation from the normal distribution of the raw or log-transformed data; hence, the nonparametric test was used. As the number of B-L3PNs found in PPC slices was small (n = 3) and membrane properties could be characterized in just 2 of these 3 B-L3PNs, these were not included in the statistical analysis. RMP, Resting membrane potential; AP threshold, voltage threshold for firing an AP; AP HW, duration, or width, of the AP at half-maximal amplitude; AHP, amplitude of the after hyperpolarizing potential; slope of f-I plot, slope of the linear portion of the relation between mean firing frequency and stimulus current.