Table 3.
Physiological parameters and blood-gas analysis of micea
| LV-shRNA-Con |
LV-shRNA-circTLK1 |
AAV-SYN-shRNA-Con |
AAV-SYN-shRNA-circTLK1 |
AAV-SYN-shRNA-TIPARP |
||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Sham (7) | tMCAO (7) | Sham (5) | tMCAO (7) | Sham (7) | tMCAO (7) | Sham (7) | tMCAO (7) | Sham (6) | tMCAO (7) | |
| Weight (g) | 23.73 ± 0.36 | 23.27 ± 0.61 | 24.16 ± 0.91 | 23.39 ± 0.55 | 23.66 ± 0.71 | 23.46 ± 0.35 | 23.77 ± 0.46 | 23.97 ± 0.77 | 23.25 ± 0.62 | 23.30 ± 0.97 |
| Body temperature (°C) | 36.73 ± 0.21 | 36.81 ± 0.17 | 36.80 ± 0.18 | 36.80 ± 0.21 | 36.76 ± 0.19 | 37.01 ± 0.09 | 36.87 ± 0.09 | 36.83 ± 0.10 | 36.68 ± 0.13 | 36.81 ± 0.16 |
| SBP (B) (mmHg) | 100.3 ± 5.2 | 94.3 ± 6.3 | 100.8 ± 4.7 | 98.6 ± 5.9 | 102.3 ± 3.6 | 107.7 ± 4.9 | 102.0 ± 2.1 | 98.2 ± 4.4 | 102.4 ± 4.1 | 97.2 ± 2.4 |
| SBP (A) (mmHg) | 95.9 ± 7.6 | 93.9 ± 6.5 | 108.8 ± 4.0 | 97.9 ± 3.5 | 99.7 ± 8.1 | 99.4 ± 5.3 | 104.0 ± 7.5 | 95.8 ± 4.1 | 99.2 ± 5.5 | 95.1 ± 4.5 |
| pH (mmHg) | 7.34 ± 0.03 | 7.34 ± 0.02 | 7.35 ± 0.02 | 7.35 ± 0.02 | 7.36 ± 0.01 | 7.35 ± 0.02 | 7.35 ± 0.03 | 7.35 ± 0.02 | 7.35 ± 0.02 | 7.35 ± 0.02 |
| pO2 (mmHg) | 177.71 ± 7.44 | 174.43 ± 8.44 | 165.80 ± 12.24 | 174.00 ± 15.10 | 167.14 ± 6.22 | 171.14 ± 14.37 | 170.30 ± 2.40 | 175.10 ± 7.08 | 169.33 ± 10.45 | 170.86 ± 4.57 |
| pCO2 (mmHg) | 37.71 ± 1.13 | 36.03 ± 2.40 | 38.76 ± 2.62 | 41.16 ± 1.44 | 40.46 ± 1.84 | 40.57 ± 2.72 | 39.89 ± 2.61 | 37.56 ± 3.34 | 37.67 ± 1.87 | 41.66 ± 2.48 |
aThe mice were subjected to 60 min of MCAO, followed by reperfusion. Body weight was measured 15 min after the onset of reperfusion or 15 min after the sham operation. Body temperature was controlled and maintained using a feedback temperature-control unit. Systolic blood pressure (SBP) was measured before ischemia (B) and 15 min after the onset of reperfusion (A). A total of 200 μl of arterial blood was collected 15 min after the onset of reperfusion for the blood-gas determination (pH, pO2, and pCO2). Data are mean ± SEM. The number of mice in each group is shown in parentheses. No significant differences in any of the parameters were observed between treatment groups.