Figure 8.

ApoE increases excitatory synaptic strength but does not change the intrinsic electrical properties of human neurons, with ApoE4 being more and ApoE2 being less efficacious than ApoE3. Data are from human neurons cultured on MEFs in the absence of serum; recombinant ApoE (10 μg/ml) or control solutions were added at D10, and neurons were analyzed at D23–25 in a double-blind manner, alongside the morphological examination performed in parallel and shown in Figure 6-1. A, B, Summary graphs of membrane capacitance (A) and resistance (B). C, Representative traces of evoked EPSCs. D, Quantification of evoked EPSC amplitudes in human neuronal cultures. E, Representative traces of mEPSCs from human neurons. F, G, Summary plots and graphs of the frequency (F) and amplitudes (G) of mEPSCs. Quantifications are shown both as cumulative probability plots and as bar diagrams (inserts). Electrophysiological recordings were done in parallel with morphological analyses (Fig. 3-1B,C). Statistical significance (*p < 0.05; ***p < 0.001) was performed with one-way ANOVA with post hoc Tukey's multiple-comparisons test for all bar diagrams, presented as means ± SEM (n = 31–38 in 3 batches for evoked EPSCs; n = 32–39 in 5 batches for mEPSCs); Kolmogorov–Smirnov test was performed for cumulative probability plots in F and G.