Figure 2.

Detection of ERS- and apoptosis-related proteins in human podocytes that stably overexpressed WT or mutant COL4A3. (A) mRNA detection of ERS related CHOP and sXBP-1 by real-time PCR. The results showed significantly increased ERS in the COL4A3, C1615Y, and c.4317delA groups compared to those of the WT group; (B) ERS-and apoptosis-related protein detection by Western blotting showed obviously increased ERS related proteins (GRP78, GRP94, PERK, and CHOP), increased apoptosis-related protein cleaved caspase 3, and decreased apoptosis-related Bcl-2 in the c.4317delA group compared the WT group; (C) Three independent repetitions of ERS- and apoptosis-related proteins expression detection experiments in different groups (^∗compared to the COL4A3-WT group, P < 0.05). ERS: Endoplasmic reticulum stress; PCR: Polymerase chain reaction; WT: Wild-type.