Table 1. The homologue proteins, spot number, primer sequence, melting temperature, product size and fold change in gene expression, are given for muskmelon plants grown under 0 (well-watered) conditions over plants grown under 7 and 14 days of water-deficit.
S.No | Homologue Proteins | *Spot No. | Primer Sequence | Tm | Product size | Relative fold change in gene expression | |
---|---|---|---|---|---|---|---|
7 days | 14 days | ||||||
1 | RNA pseudouridine synthase 7 | 4 |
F-GCCTTGTCTCAGGACTTCTTATC R-ACCCACTACCCTTGCAATATAC |
62 | 113 | 2.03 | 13.5 |
2 | Ras-related protein RABD1 | 10 |
F-CTGTGGAACTGGATGGAAAGA R-TCCATGTGCACCTCTGTAATAG |
62 | 104 | 8.75 | 7.66 |
3 | MADS-box transcription factor | 25 |
F-GAACCAAGATAGCGGAAGTAGAG R-GTCCAGCAGGTTCCATGATATT |
62 | 132 | 1.96 | 0.31 |
4 | Chromoplast-specific carotenoid-associated protein | 39 |
F-CTCTAGCCACCACTTCCATTAC R-GGTGTTCCAATGACACCTTCT |
62 | 97 | 2.01 | 9.65 |
5 | Dihydroflavonol-4-reductase | 41 |
F-CGTCAAGATGACAGGATGGATG R- GGATGATGCTGATGAAGTCCAG |
62 | 104 | 6.32 | 11.75 |
6 | 28 kDa ribonucleoprotein | 42 |
F-GAGCCAAACGAAGATGCTAAAC R-CACAGTTCCAGCCTTCTCAA |
62 | 96 | 7.39 | 13.78 |
7 | Cytochrome P450 CYP93B25 |
46 |
F-TCATTGGCCATCTCCATCTC R-CGCAAGGGACTGATCCTAAA |
62 | 108 | 2.27 | 1.3 |
8 | NADPH adrenodoxin oxidoreductase | 48 |
F-AGGAATCGTACCCAACATTAGAG R-CTCTTCAACCACCCACATACA |
62 | 99 | 0.73 | 1.21 |
*Gene ID for respective spot number are in Table 3