Fig 1. Pictorial representation of the kinetic parameterization pipeline for constructing kinetic models of metabolic networks.

(1A): A set of isotopic labeling data across a range of genetic and/or environmental conditions must be generated or procured. (1B): A stoichiometric model must be constructed. (2): ¹³C-MFA is performed, and flux ranges are elucidated using the procured isotopic labeling data across all strains from step 1A and the stoichiometric model constructed in step 1B. (3): The flux distributions that were generated in step 2 are used as training data for parameterizing the kinetic model using the stoichiometric model constructed in step 1B and the K-FIT algorithm.