Table 3. k-ecoli74 metabolite yield predictions under genetic conditions not used for parameterization and comparison with experimental values and values reported for identical strains using the k-ecoli457 model.
| Yield (moles per mole of glucose)/ Percent Overprediction |
||||||||
|---|---|---|---|---|---|---|---|---|
| Product | Fold Change in Gene Expression | Target Reaction | Experimental/ Reference |
k-ecoli74 | k-ecoli457 | |||
| Malate | 0.3x PTAr 10x PPC |
MDH | 0.15 | [69] | 0.42 | 180% | 0.84 | 460% |
| Acetate | 0.1x RPI | Acetate Exchange | 0.74 | [71] | 0.87 | 18% | 0.2 | 73% |
| L-Valine | 0.1x THRD-L | Valine Synthesis | 0.34 | [77] | 0.03 | -91% | 0.02 | -94% |
| Naringenin | ΔSUCOAS 0.1x FUM |
Tyrosine Synthesis | 0.0083 0.0085 0.0055 |
[76] | 0.011 | 33% | 0.015 | 36% |
| ΔMDH 0.1x SUCOAS |
0.0033 | [76] | 0.011 | 233% | 0.0091 | 176% | ||
| Lactic Acid | 0.1x ACKr | PGM/ENO | 1.07 | [78] | 1.55 | 45% | 1.2 | 12% |
| ΔACKr | 1.13 | [78] | 1.26 | 12% | 1.11 | -2% | ||
| 2,3-Butanediol | 5x PYK | PDH | 0.9 | [75] | 1.12 | 24% | 0.83 | -8% |
| Glucaric Acid | 5x NADTRHD | FBA | 0.13 | [70] | 0.76 | 485% | 0.36 | 177% |
Abbreviations: Δ (reaction knockout), PTAr (phosphotransacetylase), PPC (phosphoenolpyruvate carboxylase), RPI (ribose-5-phosphate isomerase), THRD-L (L-threonine deaminase), SUCOAS (succinyl-CoA synthetase), FUM (fumarase), MDH (malate dehydrogenase), ACKr (acetate kinase), PYK (pyruvate kinase), NADTRHD (NAD transhydrogenase), PGM/ENO (phosphoglycerate mutase/enolase, lumped), PDH (pyruvate dehydrogenase), FBA (fructose-bisphosphate aldolase)