Fig 5. Hepatic glucose and glycogen levels, and mRNA levels of enzymes involved in gluconeogenesis and glycogen synthesis after 3 weeks treatment with vehicle (control) or 1-triple TTA (1-tTTA) in rats.

(a) Phosphoenolpyruvate carboxykinase, cytosolic (PEPCK-C/Pck1) mRNA level, (b) phosphoenolpyruvate carboxykinase-mitochondrial (PEPCK-M/Pck2) mRNA level, (c) pyruvate carboxylase (Pc) mRNA level, (d) hepatocyte nuclear factor 4a (Hnf4a) mRNA level, (e) glucose-6 phosphatase (G6pc) mRNA level, (f) the hepatic glucose level, (g) the hepatic glycogen level, (h) glycogen synthetase 2 (Gys2) mRNA level, (i) Gys3a mRNA level, (j) glycogen synthetase 3 beta (Gsk3b) mRNA level. Gene expression levels were normalized to the house keeping gene RPLP0 and relative values are given. All values shown are means with standard deviation (n = 6–8). Statistically significant differences between means were determined using Students t-test, *p < 0.05 vs. control.