Table 2.

Primer sequences of the methylated KCNK15-AS1 gene

Gene	Primer	Product (bp)
KCNK15-AS1,M-MSP	Forward: 5′-CGTAGCTACTGCTATTATCGTTC-3′	162
Reverse: 5′-TTATGCCTTGGGCTACGTACT-3′
KCNK15-AS1,U-MSP	Forward:5′-GGTACGTATGCTTGGGCTTATG-3′	162
Reverse: 5′-ATTGGCGCTTAGCTAGGCTA-3′

Abbreviations: M, methylated; MSP, methylation-specific PCR; U, unmethylated.