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. 2019 Sep 20;12:7739–7748. doi: 10.2147/OTT.S215796

Figure 3.

Figure 3

MiR-139-5p inhibits Wnt/β-catenin signaling in CC. (A) SiHa or Hela cells were co-transfected with TOP Flash firefly luciferase plasmid, renilla luciferase plasmid, and miR-139-5p mimics (Mimics) or controls (Con). 48 hrs later, luciferase activity was determined, and relative Wnt/β-catenin activity was calculated. (B) SiHa or Hela cells were transfected with miR-139-5p mimics (Mimics) or controls (Con). 24 hrs later, qRT-PCR was performed to detect the mRNA expressions of Wnt target genes, as well as β-catenin. (C) SiHa or Hela cells were transfected with miR-139-5p mimics (Mimics) or controls (Con). 24 hrs later, Western blot analysis was performed to detect the protein expressions of Wnt target genes, as well as β-catenin. **P<0.01, ***P<0.001.