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. 2019 Sep 20;12:7739–7748. doi: 10.2147/OTT.S215796

Figure 4.

Figure 4

TCF4 is a direct target of miR-139-5p in CC. (A) Sequence of the miR-139-5p binding site with wild type (WT) TCF4 3ʹ-UTR (top) and the mutant (MT) TCF4 3ʹ-UTR sequence (bottom). (B) SiHa or Hela cells were co-transfected with miR-139-5p mimics (Mimics) or controls (Con) and luciferase reporter vectors containing the wild type of mutant TCF4 3ʹ-UTR sequences of the miR-139-5p binding sites. 48 hrs after transfection, luciferase activities were measured. (C) qRT-PCR analysis of TCF4 mRNA expression after SiHa or Hela cells was transfected with miR-139-5p mimics (Mimics) or controls (Con). (D) Western blot analysis of TCF4 protein expression after SiHa or Hela cells was transfected with miR-139-5p mimics (Mimics) or controls (Con). **P<0.01, ***P<0.001.

Abbreviation: NS, no significance.