FIG 1.
The ΔlipA mutant is attenuated during systemic infection. Bacterial burden (log10 number of CFU) in the peritoneal cavity and kidneys of mice at 16 h (T16h) after intraperitoneal infection with the WT (n = 16), ΔlipA (n = 16), or ΔlipA + lipA (n = 16) strain (A) or 72 h (T72h) after intraperitoneal infection with the WT (n = 14), ΔlipA (n = 16), or ΔlipA + lipA (n = 20) strain (B). Bars represent means. P values were determined by the Kruskal-Wallis test with Dunn’s posttest. *, P < 0.05; **, P < 0.01; ***, P < 0.001. Dashed line, limit of detection. (C) Competitive index for the lipA::erm and WT strains after coinfection (n = 12) with a 50/50 mix of the WT and lipA::erm strains for 72 h. Dashed line, competitive index of 1.