Disruption of TLR3 protein expression in human oviduct and cervical cells. The Wes simple Western system was used to confirm the disruption of TLR3 protein expression. (A) Human OE-E6/7 cells. Lane 1, ladder; lane 2, 400 ng WT human OE-E6/7 cell protein; lane 3, 400 ng hOE-TLR3KD cell protein. (B) HeLa cells. Lane 1, ladder; lane 2, 400 ng WT HeLa cell protein; lane 3, 400 ng HeLa TLR3KD cell protein. Data presented are representative data for selected TLR3-deficient clones from both cell types.