TABLE 2.
Strains and plasmids used in this study
| Strain or plasmid | Genotype or relevant characteristics | Source or reference |
|---|---|---|
| Strains | ||
| E. coli χ6212 | F− λ− ϕ80 Δ(lacZYA-argF) endA1 recA1 hsdR17 deoR thi-1 glnV44 gyrA96 relA1 ΔasdA4 | 31 |
| E. coli χ7213 | thi-1 thr-1 leuB6 fhuA21 lacY1 glnV44 ΔasdA4 recA1 RP4 2-Tc::Mu [λpir]; Kmr | 31 |
| Y. enterocolitica WA | Serotype O:8 | Robert Perry |
| Y. pseudotuberculosis PB1+ | Serotype O:1b | Robert Perry |
| Y. pseudotuberculosis χ10067 | ΔyopJ315 ΔyopK108 | 31 |
| Y. pseudotuberculosis χ10069 | Δasd206 ΔyopJ315 ΔyopK108 | This study |
| Y. pestis KIM6+(pCD1Ap) | Pgm+ pMT1 pPCP1 pCD1Ap | 65 |
| Plasmids | ||
| pRE112 | Suicide vector; Cmr mob− (RP4)R6K ori sacB | 26 |
| pYA3332 | Asd+ p15A ori | 26 |
| pYA5154 | Δasd DNA fragment of Y. pseudotuberculosis PB1+ into XmaI and KpnI sites of pRE112 | 26 |
| pYA5199 | sycE-yopE′ amino acid 1 to 138-lcrV fragment cloned into pYA3332 | 26 |