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. Author manuscript; available in PMC: 2020 Oct 1.
Published in final edited form as: J Pharm Sci. 2019 Apr 30;108(10):3443–3456. doi: 10.1016/j.xphs.2019.04.019

Fig 5. Long-lasting inhibitory effects of EVR on OATP1B1- and OATP1B3-mediated transport.

Fig 5

Model-estimated fold change and associated SE of the accumulation of [3H]-rosuvastatin (20 nM, 0.5 min) (A) and [3H]-CCK-8 (1 µM, 3 min) (B) vs. vehicle control (CTL). HEK293-OATP1B1 or –OATP1B3 cells were pre-incubated with CTL or 0.2 µM EVR-containing culture medium for 1 h. At the end of pre-incubation, the culture medium was removed and cells were washed three times with fresh culture medium and cultured in inhibitor-free medium for the indicated time periods. [3H]-rosuvastatin (20 nM, 0.5 min) (A) or [3H]CCK-8 (1 µM, 3 min) accumulation was determined at the indicated time points (n=3 in triplicate) in the absence of EVR after washing. Fold changes and SE were estimated using a generalized linear mixed model, as described in the “Materials and Methods”. * indicates a statistically significant difference (p<0.05) vs. CTL.