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. Author manuscript; available in PMC: 2020 Oct 1.
Published in final edited form as: J Pharm Sci. 2019 Apr 30;108(10):3443–3456. doi: 10.1016/j.xphs.2019.04.019

Fig. 7. Effects of dual mTOR inhibitor INK-128 on pre-incubation effects of EVR and SIR.

Fig. 7

Model-estimated fold change and associated SE of the accumulation of [3H]-E217βG (1 µM, 2 min) (A) and [3H]-CCK-8 (1 µM, 3 min) (B) in HEK293-OATP1B1 and HEK293-OATP1B3 cells, respectively. The cells were pre-incubated with vehicle CTL-, 0.2 µM EVR-, or 0.5 µM SIR-containing culture medium for 1 hour either alone or in combination with 10 µM INK-128, following an earlier 1-hour treatment with 10 µM INK-128 alone. At the end of pre-incubation, the culture medium was removed and substrate accumulation was determined (n=3 in triplicate) in the absence of inhibitors after washing. Fold changes and SE were estimated using a generalized linear mixed model, as described in the “Materials and Methods”. * indicates a statistically significant difference (p<0.05) vs. CTL.