. Author manuscript; available in PMC: 2020 Oct 1.

Published in final edited form as: J Pharm Sci. 2019 Apr 30;108(10):3443–3456. doi: 10.1016/j.xphs.2019.04.019

Table 3.

Effects of preincubation with EVR and SIR on transport kinetics of OATP1B1 and OATP1B3. K_m and V_max values of OATP1B1-mediated E₂17ßG transport and OATP1B3- mediated CCK-8 transport in vehicle control (CTL), EVR (1 μΜ, 1 h) and SIR (1 μΜ, 1 h) preincubated HEK293-OATP1B1 and -OATP1B3 cells are shown. Experiments were conducted in triplicate. Data represent mean ± SD from 3 and 5 independent experiments for OATP1B1 and OATP1B3, respectively. *, p<0.05 by one-way ANOVA followed by Dunnett’s test vs. CTL.

Transporter	K_m (µM)			V_max (pmol/mg P/min)
Transporter	CTL	EVR	SIR	CTL	EVR	SIR
OATP1B1	3.3 ± 1.3	9.7 ± 1.8*	9.2 ± 4.3*	46.9 ± 11.8	32.3 ± 9.0*	31.9 ± 5.1*
OATP1B3	5.8 ± 2.1	24.2 ± 8.7*	22.9± 8.5*	49.8 ± 16.6	39.9 ± 3.8	50.5 ± 5.3