FIGURE 6.

ZIKV infection impact on tight junction proteins expression in vivo. Brain microvessels were isolated post-mortem from ZIKV-infected immunocompetent mice at 2 dpi. Expression of ZO-1, occludin, and claudin-5 were assessed by immunoblotting in mock-infected and ZIKV-H (A), ZIKV-PR (B), and ZIKV-U (C) infected animals. ZIKV-infected animals were divided according to viral detection in the brain, according to RT-qPCR data (Figure 4) (Brain negative: ZIKV-infected mice with brain RT-qPCR negative; Brain positive: ZIKV-infected mice with brain RT-qPCR positive). GAPDH was used as an internal control. Presented blots are cropped from the originals. Data are mean ± SEM expressed as fold increase over mock-infected control. ^∗p < 0.05 vs. not infected (ANOVA + Dunnett).