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. 2019 Sep 18;10:2243. doi: 10.3389/fimmu.2019.02243

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Copyright © 2019 Huang, Wu, Chen, Peng, Li, Zhang, Zhou and Duan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The frequency of MDSCs in CRC patients. (A) The gating strategy for HLA-DR⁻CD33⁺CD11b⁺ cells from tissue samples of CRC patients. (B) Statistical analysis for the percentage of infiltrating MDSCs in 16 paired CRC samples including tumor tissues and adjacent normal tissues. (C) Representative IHC staining for CD33 in tumor tissues and adjacent normal tissues from three CRC patients. Blank scale bars = 100 μm. (D) The gating strategy for HLA-DR⁻CD33⁺CD11b⁺ cells from the peripheral blood of CRC patients. (E) Statistical analysis for the percentage of circulatory MDSCs in PBMCs from healthy controls (HC, n = 30) and CRC patients (n = 52) by FCM. (F–G) ELISA analysis of serum Arg1 and iNOS levels from HC (n = 30) and CRC patients (n = 52). Data represents the mean±SD. **p < 0.01.