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. 2019 Sep 25;7(18):e14234. doi: 10.14814/phy2.14234

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© 2019 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Expression of surface markers of activation increased on non‐stimulated CD4⁺ T cell populations. Human CD4⁺ T cells were isolated and cultured in non‐stimulated wells for 1 and 3 days then stained for CD25 and CD69 and analyzed by flow cytometry. The median fluorescence intensity (MFI) was determined for expression of CD25 at (A) 1 day (Control: 0.03 ± 0.36, Exercise: 0.3 ± 0.46) and at (B) 3 days (Control: 0.07 ± 0.7, Exercise: 0.11 ± 0.59). CD69 expression at (C) 1 days (Control: 0.25 ± 0.49, Exercise: 0.27 ± 0.54) and at (D) 3 days (Control: 0.06 ± 0.52, Exercise: 0.16 ± 0.55). Data are presented as fold change from baseline and visualized as spaghetti plots with each line representing the change between paired samples; n = 10. * Indicates a statistically significant difference (P < 0.05) from the control session.