Figure 5.

l‐homoarginine supplementation reduces glomerular macrophage infiltration in diabetic mice. (A) Glomerular macrophage recruitment was visualized by immunohistochemical staining using Mac‐2 antibody 12 weeks after l‐homoarginine supplementation. Macrophage numbers per glomerulus were counted in a blinded manner. Scale bar in images represents 10 µm. (B) The number of glomerular macrophages was counted in 20 glomeruli per section (number of macrophages in glomeruli divided by the number of glomeruli) in blinded fashion under 40× magnification and averaged. Results are presented as mean ± SEM. *P < 0.01 compared to nondiabetic control mice; ^# P < 0.05 compared to diabetic mice. N = 10 for nondiabetic, 9 for diabetes and diabetes + oral HA, 7 for diabetes + pump HA.