Table I.
Oligonucleotide primers and probes for eight SNPs of the ADIPOQ and EDN1 genes.
| A, ADIPOQ | ||||
|---|---|---|---|---|
| SNP | Direction | Sequence (5'-3') | Probe | Length (bp) |
| rs17366743 | F | GGCAGGAAAGGAGAACC | (FAM)-AGCGGTATACATAGGCACC-(RTQ1) | 180 |
| R | GTACAGCCCAGGAATGTTGC | (R6G)-CTATGTACACCGCTCAGC-(BHQ2) | ||
| rs17300539 | F | TTGAAGTTGGTGCTGGCATC | (FAM)-CAGGATCTGAGCCGGTTC-(RTQ1) | 193 |
| R | GGAAGCTGCCACCCACTTA | (R6G)-CAAGAACCAGCTCAGATCC-(BHQ2) | ||
| rs266729 | F | GTTGGTGCTGGCATC | (FAM)-CAGATCCTGCCCTTCAAA-(RTQ1) | 127 |
| R | CCTTGGACTTTCTTGGCACG | (R6G)-TGCGCTTCAAAAACAAAACAT-(BHQ2) | ||
| rs182052 | F | CCTCCGTTCTCCCAC | (FAM)-CCATTCTGAATTTTGCCCAGT-(RTQ1) | 145 |
| R | ACCCTTCCACCTTACTGACC | (R6G)-CCATTCTGAATTTTACCCAGTTCG-(BHQ2) | ||
| rs2241766 | F | GGATTCCAGGGCTCAGGATG | (FAM)-TCTGCCCGGTCATGA-(RTQ1) | 139 |
| GCCATCCAACCTGTGCAG | (R6G)-TCCTGGTCATGCCCGG-(BHQ2) | |||
| B, EDN1 | ||||
| SNP | Direction | Sequence (5'-3') | Probe | Length (bp) |
| rs2070699 | F | CTGGACATCATTTGGGTC | (FAM)-TGTAACCCTAGTCATTCATTAGCG-(RTQ1) | 145 |
| R | TGGATGGTGTTAGAAGGACTACC | (R6G)-TTGTAACCCTATTCATTCATTAGCG-(BHQ2) | ||
| rs1800542 | F | GCTAGCTCTGACTCTACTGT | (FAM)-CATGTCTCTCGGCGTT-(RTQ1) | 148 |
| R | GGACTGGGAGTGGGTTTCTC | (R6G)-CTCTCGACGTTTGAGGAGAC-(BHQ2) | ||
| rs1800543 | F | GTGATGAGCTCCTTGTGTGC | (FAM)-TAGTGTAATTAATAGTCTTTAAAAT-(RTQ1) | 182 |
| R | GGTCTGTTGCCTTTG | (R6G)-TATATTAGTGTGATTAATAGTCTT-(BHQ2) | ||
SNP, single-nucleotide polymorphism; ADIPOQ, adiponectin; EDN1, endothelin-1; F, forward; R, reverse; BHQ2, black hole quencher-2; FAM, 6-carboxyfluorescein; R6G, rhodamine 6G; RTQ1, real-time quencher-1.