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Primary antibodies, antigen retrieval solution, their dilutions, and positive area.
| Antibody | Source | Pretreated | Dilution | Positive area |
|---|---|---|---|---|
| Ki-67 (MM1) |
Leica, UK | Tris EDTA, pH9 Microwave, 20 min |
1:100 overnight |
Nucleus |
| Bcl-2 | Leica, UK | Citrate, pH6 Autoclave, 121°C, 10 min |
1:100 overnight |
Membrane and cytoplasm |
| Bax | Dako, Denmark | Citrate, pH6 Autoclave, 121°C, 10 min |
1:200 overnight |
Cytoplasm |
