FIG 5.

Inhibition of Nipah virus fusion protein budding reduces attachment protein incorporation despite the presence of normally budding matrix protein. (A) To assess the effects that manipulation of vesicular trafficking (Fig. 3) and the actin cytoskeleton (Fig. 4) have on more complete budding particles, NiV F, G, and M were cotransfected with or without DN-dynamin, DN-Vps4a, DN-Rab11, or CA-RhoA. The expression of each viral protein in cell lysates, on the cell surface for F and G, and in VLPs was quantified and used to produce budding indices as done previously. (B to D) These values are summarized for F (B), G (C), and M (D). The results are representative of at least three experiments, with error bars indicating the standard error of the mean. One-way Student t tests were used to assess significant differences in budding efficiencies for each viral protein compared to FGM coexpression alone (*, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001).