FIG 5.

Molecules produced by P. aeruginosa in patients 1 and 3. (a) The molecular network cluster of quinolones detected in the lung tissue of patients 1 and 3 and in vitro microbial cultures of Pseudomonas isolated from sputum and the swabs collected from lung sections is shown. (b) The distributions of the quinolone HHQ are shown for patients 1 and 3. All the other quinolones showed similar distributions in those patients (Fig. S8). (c) Inset views of the distribution of Pseudomonas, Staphylococcus, Granulicatella, and the Pseudomonas quinolone NHQ in patient 3 suggestive of upregulation in quinolone production by Pseudomonas in the regions where interactions of Pseudomonas with Staphylococcus and Granulicatella and possibly other microbes take place. In agreement with this observation, the levels of production of HHQ and NHQ were also found to increase in cocultures of Pseudomonas and Staphylococcus compared to Pseudomonas grown alone under identical conditions (Fig. S9).