Figure 3.

Human DDX23 plays an important role in innate immune responses to poly(I:C) stimulation and VSV infection. (A) Heatmap of RNA-seq analysis. A549 cells were transfected with siRNA (control) or with siRNA targeting DDX23 (si-DDX23) for 3 days and then uninfected or infected with VSV (MOI = 3) for 12 h. Heatmap was made by calculating log₂ [(infected RPKM)/(control RPKM)]. (B–D) ELISA of IFN-β, IL-6, and RANTES production by indicated cells stimulated with LMW poly(I:C) delivered with Lipofectamine 2,000 for 24 h. Mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001, Student's t-test. (E) Relative percentage of VSV-eGFP-infected cells (MOI = 1 or 3) transfected with indicated siRNAs 24 hpi. Mean ± SEM; *p < 0.05, **p < 0.01, Student's t-test. ^##p < 0.01 on VSV-infected si-control column compared to uninfected si-control. (F) A549 cells were transfected with the indicated siRNAs and infected with VSV-eGFP for 16 h. Viral protein was monitored by immunoblotting (MOI = 3).