Skip to main content
. 2019 Sep 18;10:2203. doi: 10.3389/fimmu.2019.02203

Figure 4.

Figure 4

CRBN inhibits the ubiquitination of ECSIT. (A) HEK293T cells were transfected with Mock, Myc-ECSIT, Flag-TRAF6, or different concentrations of Flag-CRBN, as indicated. At 38 h after transfection, transfected cells were extracted, immunoprecipitated with anti-Myc antibody, and then an IB assay was performed with anti-Flag, anti-Myc, or anti-TRAF6 antibody. (B) HEK293T cells were transfected with Mock, Flag-TRAF6, Myc-ECSIT, HA-Ub, or different concentrations of Flag-CRBN, as indicated. At 38 h after transfection, transfected cells were extracted, immunoprecipitated with anti-Myc antibody, and then an IB assay was performed with anti-Flag, anti-Myc, or anti-HA antibody. (C) Model of how CRBN inhibits the production of mROS through the inhibition of the ubiquitination of ECSIT.