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. 2019 Sep 18;10:1171. doi: 10.3389/fphys.2019.01171

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Copyright © 2019 Reynés, van Schothorst, Keijer, Ceresi, Oliver and Palou.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Schematic overview of regulated genes in tPVAT and in IAT in control (22°C) vs. cold (4°C) exposed ferrets (Student’s t-test, p-value < 0.01, with an absolute fold change ≥ 2). The arrows show the number of genes up- and down-regulated in each analysis. (B) PCA analysis of gene expression in tPVAT and IAT at 22 and 4°C of a subset of genes being differentially expressed in tPVAT vs. IAT at control (22°C) temperature (Student’s t-test, p-value < 0.01, with an absolute fold change ≥ 2). Volcano plot for all expressed probes in control (C) and cold conditions (D). Minus log10 p-value of all genes are plotted against the differences of group means (log ratio) of each gene, tPVAT vs. IAT. The line indicates the threshold p-value < 0.01. The results represent data from the control (n = 7) and cold (n = 6–7) groups.