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NO bound H-NOX inhibits HqsK kinase activity in a dose-dependent manner. Vp HqsK (D499A) was incubated with varying concentrations of NO⋅H-NOX for 30 min then another 30 min after the addition of ATP. Stained polyacrylamide gel (A) and autoradiography (B) are shown. (C) Quantified band intensities of HqsK titration with FeII-NO H-NOX. Average of three independent assays ± SEM is plotted.
