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Phosphotransfer profiling of Vp HqsK and three stand-alone Vp Hpt proteins (VP1472, VP2098, and VP2127). Stained polyacrylamide gel (A) and autoradiography (B) are shown. 1 μM each of KD and IKR were incubated with 10 μM each of Hpts. KD/IKR were loaded with phosphoryl group by preincubating with ATP for 90 min. Hpt proteins were then added to initiate the phosphotransfer. Phoshoryl group accumulation was observed only for VP2098 (LuxU) among three Hpts.
